Negative electrospray ionization tandem mass spectrometric investigation of ent-kaurane diterpenoids from the genus Isodon
稿件作者：周燕
通讯作者：丁立生，孙汉董
刊物名称：JOURNAL OF MASS SPECTROMETRY
发表年份：2008
卷：43
期：1
页码：63-73
影响因子：2.94
文章摘要：
ent-Kaurane diterpenoids are a class of natural compounds isolated from genus Isodon, which have
been found to have important bioactivities. Negative electrospray ionization tandem mass spectrometry
((−)ESI-MSn) was used to investigate the fragmentation pattern of C-20-nonoxygenated ent-kauranes
and two subtypes of C-20-oxygenated ent-kauranes by using an ion trap instrument and accurate mass
measurement on an ESI-Q-time-of-flight (TOF)mass spectrometer. The analysis revealed that loss of CH2O or CO2 is the predominant process for 7, 20-epoxy and 7, 20 : 14, 20-diepoxy subgroup of C-20-oxygenated ent-kauranes. In addition, compounds of C-20-nonoxygenated ent-kauranes with a conserved core structure but different substituent groups, such as a hydroxyl, aldehyde, carboxyl, and acetyl moiety, resulted in diagnostic product ions through losses of H2O, CO, CO2, and AcOH, respectively. This work clearly demonstrates the utility of tandem mass spectrometry for studies on the rationalization of the diagnostic fragmentation of a series of compounds from two main types of the ent-kaurane diterpenoids.
been found to have important bioactivities. Negative electrospray ionization tandem mass spectrometry
((−)ESI-MSn) was used to investigate the fragmentation pattern of C-20-nonoxygenated ent-kauranes
and two subtypes of C-20-oxygenated ent-kauranes by using an ion trap instrument and accurate mass
measurement on an ESI-Q-time-of-flight (TOF)mass spectrometer. The analysis revealed that loss of CH2O or CO2 is the predominant process for 7, 20-epoxy and 7, 20 : 14, 20-diepoxy subgroup of C-20-oxygenated ent-kauranes. In addition, compounds of C-20-nonoxygenated ent-kauranes with a conserved core structure but different substituent groups, such as a hydroxyl, aldehyde, carboxyl, and acetyl moiety, resulted in diagnostic product ions through losses of H2O, CO, CO2, and AcOH, respectively. This work clearly demonstrates the utility of tandem mass spectrometry for studies on the rationalization of the diagnostic fragmentation of a series of compounds from two main types of the ent-kaurane diterpenoids.
