Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry
稿件作者：刘鑫
通讯作者：周燕，丁立生
刊物名称：RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表年份：2008
卷：22
期：12
页码：1981-1992
影响因子：2.772
文章摘要：
A rapid, simple electrospray ionization mass spectrometry (ESI-MS) sensitivity enhancement
method for detecting carbonyl groups in triterpenoids has been developed by using hydroxylamine
hydrochloride (NH2OH·HCl) as a derivatization reagent. We use the oxime formed during the
derivatization reactions and its Beckmann rearrangement intermediates as a means of detecting
the carbonyl groups originally present in these triterpenoids. In comparison with other derivatization methods reported in the literature, this method is simple, specific and can be used to detect carbonyl groups in triterpenoids which have low polarity and are poorly or non-ionizable. Moreover, it can also be used to detect hydroxyl groups by using the Dess-Martin periodinane (DMP) to convert primary and secondary hydroxyls into carbonyl groups.
method for detecting carbonyl groups in triterpenoids has been developed by using hydroxylamine
hydrochloride (NH2OH·HCl) as a derivatization reagent. We use the oxime formed during the
derivatization reactions and its Beckmann rearrangement intermediates as a means of detecting
the carbonyl groups originally present in these triterpenoids. In comparison with other derivatization methods reported in the literature, this method is simple, specific and can be used to detect carbonyl groups in triterpenoids which have low polarity and are poorly or non-ionizable. Moreover, it can also be used to detect hydroxyl groups by using the Dess-Martin periodinane (DMP) to convert primary and secondary hydroxyls into carbonyl groups.
