Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde ehydrogenase gene from maize (Zea mays)
稿件作者：Weizao Huang,Xinrong Ma,Qilin Wang
通讯作者：
刊物名称：PLANT MOLECULAR BIOLOGY
发表年份：2008
卷：68
期：4-5
页码：451-463
影响因子：3.541
文章摘要：
Aldehyde dehydrogenases (ALDHs) play a central role in detoxification processes of aldehydes generated in plants when exposed to the stressed conditions. In order to identify genes required for the stresses responses in the grass crop Zea mays, an ALDH (ZmALDH22A1)gene was isolated and characterized. ZmALDH22A1 belongs to the family ALDH22 that is currently known only in plants. The ZmALDH22A1 encodes a protein of 593 amino acids that shares high identity with the orthologs
from Saccharum officinarum (95%), Oryza sativa (89%),Triticum aestivum (87%) and Arabidopsis thaliana (77%),respectively. Real-time PCR analysis indicates that ZmALDH22A1 is expressed differentially in different tissues.Various elevated levels of ZmALDH22A1 expression have been detected when the seedling roots exposed to abiotic stresses including dehydration, high salinity and abscisic acid (ABA). Tomato stable transformation of construct expressing the ZmALDH22A1 signal peptidefused with yellow fluorescent protein (YFP) driven by the CaMV35S-promoter reveals that the fusion protein is targeted to plastid. Transgenic tobacco plants overexpressing ZmALDH22A1 shows elevated stresses tolerance. Stresses tolerance in transgenic plants is accompanied by a reduction of malondialdehyde (MDA) derived from cellular lipid peroxidation.
from Saccharum officinarum (95%), Oryza sativa (89%),Triticum aestivum (87%) and Arabidopsis thaliana (77%),respectively. Real-time PCR analysis indicates that ZmALDH22A1 is expressed differentially in different tissues.Various elevated levels of ZmALDH22A1 expression have been detected when the seedling roots exposed to abiotic stresses including dehydration, high salinity and abscisic acid (ABA). Tomato stable transformation of construct expressing the ZmALDH22A1 signal peptidefused with yellow fluorescent protein (YFP) driven by the CaMV35S-promoter reveals that the fusion protein is targeted to plastid. Transgenic tobacco plants overexpressing ZmALDH22A1 shows elevated stresses tolerance. Stresses tolerance in transgenic plants is accompanied by a reduction of malondialdehyde (MDA) derived from cellular lipid peroxidation.
